# of Colonies
Dilution Neelo Mohsan Cullen Isha
T2 infected, Not Re-spread 4 42 4 5
T2 infected, Re-spread 10 54 10 10
10-4 Dilution 400 TNC TNC 400
10-6 Dilution 168 TNC* 168 172
Viable Cell Count
Neelo Mohsan Cullen Isha
1.68 x 10^9 cfu/ml 4.0 x 10^9 cfu/ml 1.68 x 10^9 cfu/ml 1.72 x 10^9 cfu/ml
Average* = 1.7 x 10^9 cfu/ml
Mutant Concentration
Neelo Mohsan Cullen Isha
40 mutants/ml 420 mutants/ml 40 mutant/ml 50 mutants/ml
Average* = 43 mutants/ml
Spontaneous Mutation Frequency
Neelo Mohsan
2.3 x 10^-8 mutants/ viable cell count 1.05 x 10^-7 mutants/ viable cell count
Cullen Isha
2.3x 10^-8 mutants/ viable cell count 2.9 x 10^-8 mutants/ viable cell count
Average* = 2.5 x 10^-8 mutants/ viable cell
Chi-squared value: 23.0
p value: 0.0001
Calculations*
*-For calculations involving Mohsan's cell count, we used a dummy number of 400 cells on his 10^-6 dilution plate. Due to the outlier nature of his data, and the fact that any calculations involving his viable cell count would be based on false data, we omitted his data from the calculation of averages.