Materials
1 Late exponential phase culture of wildtype E. coli
4 TBAB plates (Tryptose Blood Agar Base)
3 Sterile dilution tubes
1 Bottle of sterile dilution broth
1 spreader in ethanol
1 Bunsen Burner
Multiple pipettes
1 Incubator set at 37°C
Methods
Experimental
Transfer 0.1 ml of the E. coli culture onto the TBAB plates and then spread.
Place the plates at 37°C for two hours.
After two hours respread one of the plates.
Spray the plates with the suspension of T2.
Incubate the plates overnight.
Count the colonies.
Properly discard the plates.
Titration
Label 3 tubes (1,2,3)
To each tube add 9.9 ml of the dilution broth.
Add 0.1 ml of E. coli to tube #1 and mix.
From tube #1 take 0.1 ml and add it to tube #2 and mix.
From tube #2 take 0.1 ml and add it to tube #3 and mix.
Transfer 0.1 ml from tube #2 and put it on a TBAB plate.
Transfer 0.1 ml from tube #3 and put it on a separate TBAB plate.
Incubate for two days at 37°C.
Count the colonies.
Properly discard of the plates.